Keeping track of the hybrid components – 

CMS analyses

 
Plant breeders use the Ogura hybrid system to improve yield, resistance and vigour in rapeseed. However, to obtain the desired heterosis effects, it requires high standards of quality and purity of the hybrid system
components: CMS (cytoplasmatic male sterility), restorer, maintainer and the hybrid itself.
 
Impure CMS material causes major problems in hybrid breeding, generating low hybridity. CMS is perhaps the component in hybrid breeding that has the strongest requirements in terms of purity (often 99.9%) and
therefore in terms of quality control.
 
CMS quality control is currently carried out using different types of analyses based on individual plants. These analyses are often cost and labour intensive but can be useful in some situations. In order to achieve control of CMS purity at 99.9%, at least 1000 individuals would have to be analysed, which is often unjustifiable in terms of cost and labour. However, ScanBi Diagnostics offers analysis of CMS based on high throughput, Real-Time PCR technology. This gives significantly lower costs and a quick response.
 
ScanBi Diagnostics can provide you with sampling instructions and containers in which to place your seed or leaf samples. Send the materials for analysis and you will receive results within 5 days (earlier if needed).
 
To save costs, you can combine CMS analysis with other analyses suchas testing for GMO or pathogens, or you can have the DNA extracted for your own use.